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Monday, August 3, 2020 | History

2 edition of Chromatographic analysis of the proteolytic enzymes of the sea urchin egg found in the catalog.

Chromatographic analysis of the proteolytic enzymes of the sea urchin egg

Gunnar Lundblad

Chromatographic analysis of the proteolytic enzymes of the sea urchin egg

by Gunnar Lundblad

  • 378 Want to read
  • 36 Currently reading

Published by Almqvist and Wiksell in Stockholm, Göteborg [etc.] .
Written in English

    Subjects:
  • Proteolytic enzymes -- Analysis.,
  • Chromatographic analysis.,
  • Sea urchins -- Eggs.

  • Edition Notes

    Includes bibliographical references.

    Statement[by] Gunnar Lundblad [and others]
    SeriesArkiv för kemi,, bd. 25, nr. 37
    Classifications
    LC ClassificationsQD1 .S923 bd. 25, nr. 37, etc.
    The Physical Object
    Paginationv.
    ID Numbers
    Open LibraryOL5721771M
    LC Control Number70417607

    Abstract. The eggs of most mammals at ovulation are metabolically relatively inert cells arrested in metaphase II of meiosis. In response to an activation stimulus normally provided by the fertilizing sperm but induced artificially by a number of parthenogenetic agents, the egg resumes meiosis, undergoes a cortical reaction, and becomes metabolically more :// The sea urchin egg jelly coat (EJ) induces the acrosome reaction (AR) of sperm. We previously demonstrated that a fraction of EJ containing two glycoproteins of and kDa possess the AR

    The underlying factor in all these experiments is the matter of energy levels as indicated by oxidation-reduction potentials, and the importance of a correct potential in controlling the activation of the enzyme systems upon which growth and development of living cells depends. In order to produce a definite structure in an organism, work is ://   Division delay in sea urchin embryos induced by a specific protease inhibitor. Experimental Cell Research , 97 (1), DOI: /(76) Klaus-Dieter Jany. Studies on the digestive enzymes of the stomachless bonefish

    Abstract. The embryo develops as cells move and multiply, and the molecular and morphologic complexity of cell structures requires energy. One of the reasons for studying sea urchin egg metabolism is to translate the observed biological results of fertilization and embryonic development into quantitative chemical language (ROTHSCHILD, 19 51). This banner text can have markup.. web; books; video; audio; software; images; Toggle


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Chromatographic analysis of the proteolytic enzymes of the sea urchin egg by Gunnar Lundblad Download PDF EPUB FB2

Immunohistochemical staining of the anterior intestine sections of short‐spined sea urchin is shown in Fig. the anterior intestine, the antibody stained granules situated near the lumen of the intestine (Fig.

4B). These granules were stained intensely by methylene blue‐Azure II (Fig. 4A), but appeared more diffuse, less grouped, than in the stomach (Fig. Read "Proteolytic enzymes in sea urchin eggs: Characterization, localization and activity before and after fertilization, Journal of Cellular Physiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your :// Sea urchin eggs are metabolically (biochemically) dormant and undergo an explosive metabolic activation after fusing with a sperm.

Protein kinases play key roles in egg activation (Townley et al., ). In this laboratory we will observe some of the phenomena associated with sea urchin sperm–egg interaction (fertilization) and egg :// By aid of chromatographic assay the glucosamine content has been determined in different stages of development of the sea urchin Paracentrotus lividus.

Some data are also given for eggs of Arbacia lixula and Echinus esculentus. There is a general trend towards a decrease of the glucosamine content per egg or embryo during the :// In this study, we found that Mn influences the MMP-mediated proteolytic activity of sea urchin embryos, inhibiting the total gelatin-auto-cleaving activity of MMPs, also referred to as proteolytic /_General_considerations_for_proteolytic_cascades.

Termination of transcription is a key process in the regulation of mitochondrial gene expression in animal cells. To investigate transcription termination in sea urchin mitochondria, we cloned the mitochondrial RNA polymerase (mtRNAP) of Paracentrotus lividus and used a recombinant form of the enzyme in a reconstituted transcription system, in the presence of the DNA-binding protein :// View embryo_book_optimized from NATSCI ZOO 4 at University of Southeastern Philippines.

The Sea Urchin leffHardin Introduction The sea urchin embryo has been used for more than a century to study Proteomic analysis of aortae from human lipoprotein(a) transgenic mice shows an early metabolic response independent of atherosclerosis. PLoS ONE, 7(1), e doi: / Garama, D., Bremer, P., & Carne, A.

Extraction and analysis of carotenoids from the New Zealand sea urchin Evechinus chloroticus ://?id= Abstract. By using Artemia chorion as a specific substrate, the hatching enzyme from Artemia salina (AHE) was purified by gel-filtration and ion-exchange chromatography, and characterized biochemically and enzymatically in this study.

It was found that the AHE had a molecular weight of kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and often contained kDa The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with % identity.

In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age :// Consider the round sea urchin egg, the elongated insect egg, and the well, the classically egg shaped bird egg.

The sizes range enormously as well: mouse and human eggs are about 80– μm in diameter, frog eggs are 1 mm, emu eggs are 15 cm (really, I have one on my desk), and the elephant or whale egg—only ://   The natural substrate of sea urchin envelysin is the fertilization envelope (Figure ).The fertilization envelope forms from the vitelline layer following fertilization(Kay & Shapiro, ) and provides a definitive barrier to polyspermy and protects the fertilization envelope is a perfectly transparent balloon around the egg, a giant molecular structure of about μm baxandall j, perlmann p, afzelius ba.

immuno-electron microscope analysis of the surface layers of the unfertilised sea urchin egg. effects of the antisera on the cell ultrastructure. j cell biol. dec; – [pmc free article] graziano kd, metz ://   However, the sea urchin hatching enzyme envelysin (Chapter ) belongs to the matrix metalloprotease family (M10) (LePage & Gache, ).

The proteolytic properties of mammalian hatching enzymes are not yet clear, but they are suggested to be trypsin-like enzymes (Perona & Wassarman, ; Sawada et al., ). Respiratory behaviour of sea-urchin spermatozoa. Effect of pH and egg water on the respiratory rate.

Journal of Experimental Zoology(3), DOI: /jez Shigenori Emi, Dirck V. Myers, Guillermo A. ://   THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 3, Issue of Febru pp.

Printed m U.S A. Speract PURIFICATION AND CHARACTERIZATION OF A PEPTIDE ASSOCIATED WITH EGGS THAT ACTIVATES SPERMATOZOA* (Received for publication, September 2, ) J.

Randall Hansbrough and David L. Garbers From the Department of Pharmacology and Proteolytic Enzymes Proteolytic Enzymes Fruton, J S; Mycek, M J By JOSEPH S. FRUTON AND MARY J.

MYCEK EXOPEPTIDASES Department of Biochemistry, Yale University, New Haven, Connecticut Leucine aminopeptidase.-Most of the earlier studies on the purification and properties of this enzyme have referred to the leucine aminopeptidase of intestinal :// Первый в мире пиратский ресурс, который открыл массовый доступ к десяткам миллионов научных статей Научные статьи – публикации в специальных   Proteases of the eggs of the Moroccan locust (Doeiostaurus maroccanus Thnbg.).

Enzymolo LUNDBLAD G. & GOrIL B. () A study of cathepsins in extracts of Brissopsis ovaries. Ark. K LUNDBLAD G., LUNDBLAD M., IMMERS J.

& SHILLING W. () Chromatographic analysis of the proteolytic enzymes of the sea urchin :// Analysis by 2D zymography of the digestive enzymes extracted from Munida hepatopancreas, showed the presence of several isotrypsin- and isochymotrypsin-like enzymes in the range of kDa and 4.

Abstract. Sea urchin hybrids have often been used to study nucleo-cytoplasmic interaction during embryonic development. In diploid hybrids the sperm nucleus of one species is introduced into the egg of another species, while in haploid merogonic hybrids anucleate egg fragments are ://The sea urchin embryo is a classical model system for studying the role of the cytoskeleton in such events as fertilization, mitosis, cleavage, cell migration and ://Cloning strategy of the sea urchin mtRNAP cDNA.

The bp ORF is represented by a thick line, the untranslated sequences by thin lines. Below the complete cDNA are positioned the cDNA fragments isolated by PCR on the cDNA library with degenerate, inosine-containing primers and vector- or internal-specific primers (striped bars); the fragment obtained by hybridization-screening of the cDNA